The Blyscan™ Glycosaminoglycan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).

Test material includes:

• Cell culture derived material
• Soluble sGAGs from fluids, such as synovial fluid, urine.
• Cartilage
• Connective tissue / tumours

Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction.

The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.

The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.

Please note:

The assay is not suitable for small sulfated disaccharide fragments or for the samples containing alginates, as these contain uronic acid.

Biocolor向全球的大学和医学研究机构分发生命科学检测方法。

我们的测定使用与天然聚合物结合的染料，例如复杂的碳水化合物，肽和蛋白质。该方法通常需要分离靶分子或选择性地改变染料结合反应条件。我们已经针对微克检测和测量水平开发了一系列新颖的检测方法。